Light and electron microscopical observations of the effects of high-density lipoprotein on growth of Plasmodium falciparum in vitro

Author Posting. © Cambridge University Press, 2004. This article is posted here by permission of Cambridge University Press for personal use, not for redistribution. The definitive version was published in Parasitology 128 (2004): 577-584, doi:10.1017/S0031182004005025.Human serum high-density lipoprotein (HDL) is necessary and sufficient for the short-term maintenance of Plasmodium falciparum in in vitro culture. However, at high concentrations it is toxic to the parasite. A heat-labile component is apparently responsible for the stage-specific toxicity to parasites within infected erythrocytes 12–42 h after invasion, i.e. during trophozoite maturation. The effects of HDL on parasite metabolism (as determined by nucleic acid synthesis) are evident at about 30 h after invasion. Parasites treated with HDL show gross abnormalities by light and electron microscopy.Professor Hajduk was supported by NIH. Professor Day was supported by a Research Leave Fellowship from The Wellcome Trust. Dr Imrie and Ms Carter were supported by Programme Grant funding awarded to Professor Day from The Wellcome Trust. Dr Ferguson was supported by an equipment grant from The Wellcome Trust

Distributed low voltage power supply system for front end electronics of the TRT detector in ATLAS experiment

We present a low voltage power supply system which has to deliver to the front end electronics of the ATLAS TRT detector [1] ca. 23 kW of electrical power over the distance of 55 -106 m (which adds another 24 kW). The system has to operate in magnetic field and under radiation environment of the LHC experimental cavern. The system has ~ 3000 individual channels which are all monitored and controlled (voltage and current measurement). The hardware solutions are described as well as the system control software

Triton calculations with π\pi and ρ\rho exchange three-nucleon forces

The Faddeev equations are solved in momentum space for the trinucleon bound state with the new Tucson-Melbourne $\pi$ and $\rho$ exchange three-nucleon potentials. The three-nucleon potentials are combined with a variety of realistic two-nucleon potentials. The dependence of the triton binding energy on the $\pi NN$ cut-off parameter in the three-nucleon potentials is studied and found to be reduced compared to the case with pure $\pi$ exchange. The $\rho$ exchange parts of the three-nucleon potential yield an overall repulsive effect. When the recommended parameters are employed, the calculated triton binding energy turns out to be very close to its experimental value. Expectation values of various components of the three-nucleon potential are given to illustrate their significance for binding.Comment: 17 pages Revtex 3.0, 4 figures. Accepted for publication in Phys. Rev.

The Real-Time Evolution of Sakurai's Star (V4334 Sgr) and other (V)LTP Objects

We report on the progress of our on-going campaign to monitor the evolution of the VLTP objects V4334 Sgr and V605 Aql, as well as the suspected (V)LTP object CK Vul. V4334 Sgr does not show signs of increased ionization compared to our previous observations in 2004. We obtained the first radio detection of V605 Aql, indicating a strong increase in radio flux since 1987. We also present the first radio detection of CK Vul and discuss the expansion of the material ejected during the 1670 event.Comment: 4 pages, 2 figures in eps format, IAU symp. 234: `Planetary Nebulae in our Galaxy and Beyond', eds. M. J. Barlow & R. H. Mende

Three-Nucleon Force and the Δ\Delta-Mechanism for Pion Production and Pion Absorption

The description of the three-nucleon system in terms of nucleon and $\Delta$ degrees of freedom is extended to allow for explicit pion production (absorption) from single dynamic $\Delta$ de-excitation (excitation) processes. This mechanism yields an energy dependent effective three-body hamiltonean. The Faddeev equations for the trinucleon bound state are solved with a force model that has already been tested in the two-nucleon system above pion-production threshold. The binding energy and other bound state properties are calculated. The contribution to the effective three-nucleon force arising from the pionic degrees of freedom is evaluated. The validity of previous coupled-channel calculations with explicit but stable $\Delta$ isobar components in the wavefunction is studied.Comment: 23 pages in Revtex 3.0, 9 figures (not included, available as postscript files upon request), CEBAF-TH-93-0

Anisotropic elasticity in confocal studies of colloidal crystals

We consider the theory of fluctuations of a colloidal solid observed in a confocal slice. For a cubic crystal we study the evolution of the projected elastic properties as a function of the anisotropy of the crystal using numerical methods based on the fast Fourier transform. In certain situations of high symmetry we find exact analytic results for the projected fluctuations.Comment: 6 pages, 7 figure

Relativistic Hamiltonians in many-body theories

We discuss the description of a many-body nuclear system using Hamiltonians that contain the nucleon relativistic kinetic energy and potentials with relativistic corrections. Through the Foldy-Wouthuysen transformation, the field theoretical problem of interacting nucleons and mesons is mapped to an equivalent one in terms of relativistic potentials, which are then expanded at some order in 1/m_N. The formalism is applied to the Hartree problem in nuclear matter, showing how the results of the relativistic mean field theory can be recovered over a wide range of densities.Comment: 14 pages, uses REVTeX and epsfig, 3 postscript figures; a postscript version of the paper is available by anonymous ftp at ftp://carmen.to.infn.it/pub/depace/papers/951

Individual variation in levels of haptoglobin-related protein in children from Gabon

Background: Haptoglobin related protein (Hpr) is a key component of trypanosome lytic factors (TLF), a subset of highdensity lipoproteins (HDL) that form the first line of human defence against African trypanosomes. Hpr, like haptoglobin (Hp) can bind to hemoglobin (Hb) and it is the Hpr-Hb complexes which bind to these parasites allowing uptake of TLF. This unique form of innate immunity is primate-specific. To date, there have been no population studies of plasma levels of Hpr, particularly in relation to hemolysis and a high prevalence of ahaptoglobinemia as found in malaria endemic areas. Methods and Principal Findings: We developed a specific enzyme-linked immunosorbent assay to measure levels of plasma Hpr in Gabonese children sampled during a period of seasonal malaria transmission when acute phase responses (APR), malaria infection and associated hemolysis were prevalent. Median Hpr concentration was 0.28 mg/ml (range 0.03-1.1). This was 5-fold higher than that found in Caucasian children (0.049 mg/ml, range 0.002-0.26) with no evidence of an APR. A general linear model was used to investigate associations between Hpr levels, host polymorphisms, parasitological factors and the acute phase proteins, Hp, C-reactive protein (CRP) and albumin. Levels of Hpr were associated with Hp genotype, decreased with age and were higher in females. Hpr concentration was strongly correlated with that of Hp, but not CRP

Manifestation of three-body forces in three-body Bethe-Salpeter and light-front equations

Bethe-Salpeter and light-front bound state equations for three scalar particles interacting by scalar exchange-bosons are solved in ladder truncation. In contrast to two-body systems, the three-body binding energies obtained in these two approaches differ significantly from each other: the ladder kernel in light-front dynamics underbinds by approximately a factor of two compared to the ladder Bethe-Salpeter equation. By taking into account three-body forces in the light-front approach, generated by two exchange-bosons in flight, we find that most of this difference disappears; for small exchange masses, the obtained binding energies coincide with each other.Comment: 24 pages, 8 figures, submitted in Few-Body System